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C19orf51 Rabbit Polyclonal Antibody
SKU: orb182488
Featured
Description
Images & Validation
−Item 1 of 4
| Tested Applications | IF, IHC-Fr, IHC-P, WB |
|---|---|
| Dilution range | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Equine, Human, Porcine |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from human C19orf51 (151-250/541aa) |
| Target | DNAAF3 |
| Molecular Weight | 59 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Disclaimer | For research use only |
Alternative Names
−C19orf51; DAAF3_HUMAN; DNAAF3; Dynein assembly factor 3, axonemal; FLJ36139; FLJ40069; UPF0470 protein C19orf51.
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Sample: Cerebellum (Mouse) Lysate at 40 ug, Primary: Anti-C19orf51 (orb182488) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 59 kD, Observed band size: 64 kD.
Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-C19orf51 Polyclonal Antibody, Unconjugated (orb182488) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-C19orf51 Polyclonal Antibody, Unconjugated (orb182488) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
Tissue/Cell: rat testis tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-C19orf51 Polyclonal Antibody, Unconjugated (orb182488) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
Quick Database Links
Gene Symbol
DNAAF3
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
IF
Immunofluorescence
C19orf51 Rabbit Polyclonal Antibody (orb182488)
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