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Caspase-8 subunit p18 Rabbit Polyclonal Antibody
SKU: orb1676
Featured
Description
Research Area
Apoptosis, Cancer Research, Caspases, Cell Biology, Cell Cycle, Metabolism, Necroptosis Pathway
Images & Validation
−Item 1 of 3
| Tested Applications | FC, IF, IHC-Fr, IHC-P, WB |
|---|---|
| Dilution Range | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg /Test |
| Reactivity | Human, Rat |
| Predicted Reactivity | Bovine, Canine, Equine, Mouse, Porcine |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from human caspase-8 subunit p18 (188-280/479aa) |
| Target | CASP8 |
| Molecular Weight | 18/55 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Caspase-8 subunit p18; ALPS2B; Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 12 protein; Apoptosis related cysteine peptidase; Apoptotic cysteine protease; Apoptotic protease Mch 5; Apoptotic protease Mch-5; Apoptotic protease Mch5; CAP 4; CAP4; CASP 8; CASP-8; CASP8; CASP8_HUMAN; Caspase 8; Caspase 8 apoptosis related cysteine peptidase; Caspase-8 subunit p18; Caspase8; CED 3; FADD homologous ICE/CED 3 like protease; FADD Homologous ICE/CED3 Like Protease; FADD Like ICE; FADD-homologous ICE/CED-3-like protease; FADD-like ICE; FLICE; FLJ17672; ICE like apoptotic protease 5; ICE-like apoptotic protease 5; MACH alpha 1/2/3 protein; MACH; MACH beta 1/2/3/4 protein; MCH 5; MCH5; MGC78473; MORT1 associated CED 3 homolog; MORT1 associated CED3 homolog; MORT1-associated CED-3 homolog; OTTHUMP00000163717; OTTHUMP00000163720; OTTHUMP00000163724; OTTHUMP00000163725; OTTHUMP00000165062; OTTHUMP00000165063; OTTHUMP00000165064; OTTHUMP00000206552; OTTHUMP00000206582
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Blank control (blue line): U251 (fixed with 70% ethanol overnight at 4°C and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature). Primary Antibody (green line): Rabbit Anti-caspase-8 subunit p18 antibody (orb1676), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, dilution: 1 µg/Test.
Paraformaldehyde-fixed, paraffin embedded (Rat liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (caspase-8 subunit p18) Polyclonal Antibody, Unconjugated (orb1676) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Sample: Jurkat (Human) Cell Lysate at 30 ug, K562 (Human) Cell Lysate at 30 ug, U87MG (Human) Cell Lysate at 30 ug, Primary: Anti-caspase-8 subunit p18 (orb1676) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 55/18 kD, Observed band size: 57 kD.
Quick Database Links
Gene Symbol
CASP8
UniProt
UniProt Details
− No UniProt data available
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
FC
Flow Cytometry
IF
Immunofluorescence
Caspase-8 subunit p18 Rabbit Polyclonal Antibody (orb1676)
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