CCL8 Rabbit Polyclonal Antibody

SKU: orb592724

Description

Rabbit polyclonal antibody to CCL8

Research Area

Cell Biology, Immunology & Inflammation, Signal Transduction

Images & Validation

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Item 1 of 4

Tested Applications	IHC, WB
Reactivity	Human
Predicted Reactivity	Human

Related Conjugates & Formulations

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Biotin

FITC

HRP

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Immunogen	The immunogen is a synthetic peptide directed towards the middle region of human CCL8
Target	CCL8
Molecular Weight	11 kDa
Purification	Affinity Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration	0.5 mg/ml
Disclaimer	For research use only

Alternative Names

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HC14, MCP2, MCP-2, SCYA8, SCYA10

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25 ug of the indicated Human whole cell extracts was loaded onto a 10-20% SDS-PAGE gel. 1 ug/ml of the antibody was used in this experiment.

Positive control (+): Human Fetal Heart (HE), Negative control (-): HeLa Cell Lysate (HL), Antibody concentration: 1 ug/ml.

Rabbit Anti-CCL8 antibody, Formalin Fixed Paraffin Embedded Tissue: Human Adrenal, Primary antibody Concentration: 1:100, Secondary Antibody: Donkey anti-Rabbit-Cy3, Secondary Antibody Concentration: 1:200, Magnification: 20x, Exposure Time: 0.5-2.0 sec.

WB Suggested Anti-CCL8 Antibody Titration: 0.2-1 ug/ml, ELISA Titer: 1:62500, Positive Control: Human heart.

Quick Database Links

Gene Symbol

CCL8

UniProt

P80075

RefSeq (Protein):NP_005614

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_005614

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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