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CD171/L1CAM Recombinant Rabbit Monoclonal Antibody
SKU: orb1600350
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Description
Images & Validation
−Item 1 of 2
| Tested Applications | IF, IHC-Fr, IHC-P, WB |
|---|---|
| Dilution range | WB=1:1000-5000, IHC-P=1:200-800, IHC-F=1:200-800, IF=1:200-800 |
| Reactivity | Human |
| Predicted Reactivity | Human |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Recombinant |
| Isotype | IgG |
| Clone No. | 1C3 |
| Immunogen | A synthesized peptide derived from human NCAM L1 (900-1150/1257aa) |
| Target | L1CAM |
| Molecular Weight | 138 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 1*TBS (pH7.4), 0.05% rAlbumin, 40% Glycerol. Preservative: 0.02% Proclin300. |
| Concentration | 1mg/ml |
| Disclaimer | For research use only |
Alternative Names
−L1CAM_HUMAN; Neural cell adhesion molecule L1; CAML1; MIC5; N-CAM-L1; NCAM-L1; CD171; L1 cell adhesion molecule; S10; HSAS; MASA; SPG1; HSAS1; N-CAM-L1;
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Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-L1CAM antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Western blot analysis of L1CAM on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate, Lane 2: Human brain tissue lysate.
Quick Database Links
Gene Symbol
L1CAM
UniProt
UniProt Details
− No UniProt data available
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
IF
Immunofluorescence
CD171/L1CAM Recombinant Rabbit Monoclonal Antibody (orb1600350)
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