Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human CD40L (31-150/261aa)
Target	CD40LG
Molecular Weight	32-39 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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TNFSF5; CD 154; CD154; CD 40L; CD154 antigen; CD40 homologue; CD40 ligand; CD40LG; gp39; hCD40L; HIGM1; Hyper IgM syndrome; IGM; IMD3; Ly62; T B cell activating molecule; T cell antigen GP39; TBAM; TNF related activation protein; TNF superfamily member 5; Tnfsf5; TRAP; Tumor necrosis factor(ligand) superfamily member 5; CD40L_HUMAN.

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Paraformaldehyde-fixed, paraffin embedded (human liver cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CD40L) Polyclonal Antibody, Unconjugated (orb10326) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat spleen), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CD40L) Polyclonal Antibody, Unconjugated (orb10326) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Quick Database Links

Gene Symbol

CD40LG

UniProt

P29965

UniProt Details

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No UniProt data available

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Protocol Information

IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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