CD80 Rabbit Polyclonal Antibody

SKU: orb1675375

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Description

Images & Validation

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Item 1 of 3

Tested Applications	ELISA, WB
Dilution range	WB=1:500-2000, ELISA=1:5000-10000
Reactivity	Human, Mouse
Predicted Reactivity	Rat

Related Conjugates & Formulations

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Biotin

HRP

IRDye800

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Recombinant mouse CD80 protein (38-246/306aa)
Target	CD80
Molecular Weight	32 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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Activation B7-1 antigen; B lymphocyte activation antigen B7; B7; B7-1; B7-1 antigen; BB1; CD28 antigen ligand 1; CD28LG; CD28LG1; CD80 antigen(CD28 antigen ligand 1, B7-1 antigen); CD80 antigen; CD80 molecule; CD80_HUMAN; Costimulatory factor CD80; costimulatory molecule variant IgV-CD80; CTLA-4 counter-receptor B7.1; LAB7; T-lymphocyte activation antigen CD80.

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Sample: Lane 1: Mouse Lung tissue lysates, Lane 2: Human HL-60 cell lysates, Primary: Anti-CD80 (orb1675375) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 32 kDa, Observed band size: 68 kDa.

Measured by its binding ability in a indirect ELISA. Immobilized Mouse CD80 protein, His Tag at 2 µg/mL (100 µl/well) can bind Rabbit Anti-Mouse CD80 Antibody, the EC50 is 36.63 ng/ml.

Measured by its binding ability in a indirect ELISA. Immobilized Mouse CD80 protein, His Tag at 2 µg/mL (100 µl/well) can bind Rabbit Anti-Mouse CD80 Antibody, the EC50 is 31.10 ng/ml.

Quick Database Links

Gene Symbol

CD80

UniProt

Q00609

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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