Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from rat CD80 (251-321/321aa)
Target	CD80
Molecular Weight	60-65 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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Activation B7-1 antigen; B lymphocyte activation antigen B7; B7; B7-1; B7-1 antigen; BB1; CD28 antigen ligand 1; CD28LG; CD28LG1; CD80; CD80 antigen(CD28 antigen ligand 1, B7-1 antigen); CD80 antigen; CD80 molecule; CD80_HUMAN; Costimulatory factor CD80; costimulatory molecule variant IgV-CD80; CTLA-4 counter-receptor B7.1; LAB7; T-lymphocyte activation antigen CD80; CD80_RAT.

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Sample: Lane 1: Raw264.7 (Mouse) Cell Lysate at 30 ug, Lane 2: SP2/0 (Mouse) Cell Lysate at 30 ug, Lane 3: Thymus (Rat) Lysate at 40 ug, Lane 4: Raji (Human) Cell Lysate at 30 ug, Lane 5: K562 (Human) Cell Lysate at 30 ug, Primary: Anti-CD80 (orb5805) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 60-65 kD, Observed band size: 60 kD.

Sample: Lane 1: Raw264.7 (Mouse) Cell Lysate at 30 ug, Lane 2: SP2/0 (Mouse) Cell Lysate at 30 ug, Lane 3: Thymus (Rat) Lysate at 40 ug, Lane 4: Raji (Human) Cell Lysate at 30 ug, Lane 5: K562 (Human) Cell Lysate at 30 ug, Primary: Anti-CD80 at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 60-65 kD, Observed band size: 60 kD.

Quick Database Links

Gene Symbol

CD80

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Protocol Information

Western Blot (IB, immunoblot)

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IHC (1)

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Mouse (1)

SP Dai, CC Yang, Y Chin, WH Sun TDAG8-mediated distinct signaling pathways modulate the early and late phases of neuropathic pain iScience, (2024)

PubMed:

Applications

IHC

Reactivity

Mouse