CD9 Rabbit Polyclonal Antibody

SKU: orb499626

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Description

Images & Validation

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Tested Applications	FC, IF, IHC-Fr, IHC-P
Dilution range	IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test
Reactivity	Human, Mouse
Predicted Reactivity	Bovine, Rabbit, Rat, Sheep

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human CD9 (101-200/228aa)
Target	CD9
Molecular Weight	25 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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Tetraspanin 29; 5H9; 5H9 antigen; Antigen defined by monoclonal; 602 29; Antigen defined by monoclonal; 60229; BA 2; BA2; BTCC 1; BTCC1; CD 9; CD9; CD9 antigen; CD9 antigen p24; CD9 molecule; CD9_HUMAN; Cell growth inhibiting gene 2 protein; Cell growth-inhibiting gene 2 protein; DRAP 27; DRAP27; GIG 2; GIG2; Growth inhibiting gene 2 protein; Leukocyte antigen MIC3; MIC 3; MIC3; Motility related protein; Motility-related protein; MRP 1; MRP-1; MRP1; p24; P24; p24 antigen; Tetraspanin 29; Tetraspanin-29; Tetraspanin29; Tspan 29; Tspan-29; Tspan29.

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Blank control: MCF-7. Primary Antibody (green line): Rabbit Anti-CD9 antibody (orb499626), dilution: 1 ug/Test, Secondary Antibody (green line): Goat anti-rabbit IgG-AF488, dilution: 0.5 ug/Test. Negative control (white blue line): PBS, Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CD9) Polyclonal Antibody, Unconjugated (orb499626) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Quick Database Links

Gene Symbol

CD9

UniProt

P21926

UniProt Details

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No UniProt data available

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Protocol Information

IHC-P

Immunohistochemistry Paraffin

View Protocol

IHC-Fr

Immunohistochemistry Frozen

Flow Cytometry

Immunofluorescence