Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human CFC1 (1-100/223aa)
Target	CFC1
Molecular Weight	15 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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CFC 1; CFC1; CFC1_HUMAN; CFC1B; CR 1; Cripto 1; Cripto; Cripto FRL 1 cryptic family 1; CRYPTIC; Cryptic family 1; Cryptic family protein 1; Cryptic gene; Cryptic protein; DTGA2; FLJ77897; FRL 1; HTX2; MGC133213.

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Paraformaldehyde-fixed, paraffin embedded (Human glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CFC1) Polyclonal Antibody, Unconjugated (orb182563) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Protein: Hela (human) lysate at 40 ug, Primary: rabbit Anti-CFC1 (orb182563) at 1:300, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1:5000, Predicted band size: 15 kD, Observed band size: 15 kD.

Quick Database Links

Gene Symbol

CFC1

UniProt

P0CG37

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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