Cholesterol (animal-free)

SKU: orb1310316

Description

Cholesterol is a fundamental mammalian sterol constituting 20–25% of the plasma membrane, where it modulates fluidity and permeability. As an endogenous ERRα agonist and precursor for hormones and bile acids, it is integral to metabolic studies and is commonly employed in hyperlipidemia research models, both in vitro and in vivo.

Research Area

Metabolism Research

Images & Validation

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Item 1 of 1

Key Properties

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CAS Number	57-88-5
MW	386.65
Purity	99.94% (May vary between batches)
Formula	C₂₇H₄₆O
SMILES	C[C@@]12[C@]([C@]3([C@@]([C@]4(C)C(=CC3)C[C@@H](O)CC4)(CC1)[H])[H])(CC[C@@]2([C@@H](CCCC(C)C)C)[H])[H]
Target	Estrogen Receptor/ERR,Liposome,Antibacterial,Endogenous Metabolite,MRP,ROR
Solubility	H2O:< 1 mg/mL (insoluble);Ethanol:11.42 mg/mL (29.54 mM);DMSO:< 1mg/mL (insoluble)

Bioactivity

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In Vivo

METHODS: To induce hypercholesterolemia, STD:ddY mice were fed a high cholesterol diet (1% cholesterol, 0.5% cholic acid, 0.5% olive oil and 93% standard mouse chow). RESULTS: Cholesterol can be used to construct a mouse model of hypercholesterolemia. METHODS: To induce hyperlipidemia, CD-1 mice were fed a high cholesterol diet (2% cholesterol and 0.6% sodium deoxycholate). RESULTS: Cholesterol can be used to construct a mouse model of hyperlipidemia.

In Vitro

METHODS: CD4+ T lymphocytes were incubated with 7-KC (17.5-70 µM) and Cholesterol-MβCD (17.5-70 µM) for 10 min, and T cell membrane order and disorder were assessed using di-4 ANEPPDHQ fluorescent dye. RESULTS: After exposure to 7-KC, T cell membrane order was altered in a dose-dependent manner, with significant reconstitution of membrane order observed only in cells treated with 35 µM Cholesterol, while reconstitution with l7.5 µM Cholesterol induced minimal effects. METHODS: Human gastric cancer cells SNU601, SNU638 and SNU216 were treated with Cholesterol (25-100 µM) for 48 h and cell viability was measured using MTT Assay. RESULTS: Cholesterol caused a dose-dependent decrease in cell viability in all three cell lines.

Storage & Handling

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Storage	store at low temperature,store under nitrogen \| Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice/Shipping at ambient temperature.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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signaling, phosphorylation, RORα, SREBP2, transporters, unesterified, inhibit, MRP1, IRS-1, insulin, Inhibitor, Endogenous Metabolite, EndogenousMetabolite, exocytic, ERR, ERRα, Estrogen Receptor, EstrogenReceptor, Estrogen Receptor/ERR, Diabetes, Cholesteryl alcohol, Cholesterin, Cholesterol, AKT

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Chemical structure of orb1310316

Compound Identifiers

PubChem CID

304 ↗

Key Properties

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No computed properties available.

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