CIAP1 Rabbit Polyclonal Antibody

SKU: orb100469

This product can be custom made

Featured

Description

CIAP1 Rabbit Polyclonal Antibody

Research Area

Apoptosis, Cancer Research, Caspases, Cell Cycle, Hypoxia, Metabolism, Necroptosis Pathway, Survivin / Inhibitor of Apoptosis (IAP)

Images & Validation

−

Item 1 of 3

Tested Applications	IF, IHC-Fr, IHC-P, WB
Dilution Range	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Reactivity	Mouse, Rat
Predicted Reactivity	Human

Related Conjugates & Formulations

−

Key Properties

−

Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human cIAP1 (51-150/618aa)
Target	BIRC2
Molecular Weight	61,72 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

−

Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

−

API1; HIAP2; Hiap-2; MIHB; RNF48; c-IAP1; cIAP1; Api2; Birc3; C330006D17Rik; HIAP1; IAP1; IAP2; MIAP1; MIAP2; MIHC; cIAP2; mcIAP1; rIAP1; BIRC2_HUMAN; BIRC2; Cellular inhibitor of apoptosis 1 (C-IAP1); IAP homolog B; Inhibitor of apoptosis protein 2 (hIAP-2 | hIAP2); RING finger protein 48; RING-type E3 ubiquitin transferase BIRC2; TNFR2-TRAF-signaling complex protein 2; 2.3.2.27; BIRC2_MOUSE; Inhibitor of apoptosis protein 2 (mIAP2);

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Paraformaldehyde-fixed, paraffin embedded (mouse testis tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (cIAP1) Polyclonal Antibody, Unconjugated (orb100469) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat heart tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (cIAP1) Polyclonal Antibody, Unconjugated (orb100469) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Sample: Lane 1: Mouse Testis tissue lysates, Lane 2: Rat Testis tissue lysates, Primary: Anti-cIAP1 (orb100469) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 72 kDa, Observed band size: 61, 72 kDa.

Quick Database Links

Gene Symbol

BIRC2

UniProt

Q13490

UniProt Details

−

No UniProt data available

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

Western Blot (IB, immunoblot)

View Protocol

IHC-P

Immunohistochemistry Paraffin

View Protocol

IHC-Fr

Immunohistochemistry Frozen

View Protocol

Immunofluorescence

View Protocol

Find More Protocols