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COL2A1 antibody
SKU: orb10436
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Description
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−Item 1 of 14
| Tested Applications | ELISA, ICC, IF, IHC-P, WB |
|---|---|
| Dilution range | WB:1:200, IF/ICC: 1:300, IHC-P:1:300 (based on 0.5 mg/ml) |
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from human COL2A1. Please contact us for the exact immunogen sequence. The peptide is available as orb374716. |
| Target | COL2A1 |
| Molecular Weight | 117 kDa |
| Purity | Polyclonal antibodies are purified by peptide affinity chromatography |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | 10 mM PBS, 0.02% sodium azide |
| Concentration | - 100 μg (in 200 μl): 0.5 mg/ml- 200 μg (in 400 μl): 0.5 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−anti-CB11 antibody, anti-COL11A3 antibody, anti-Dmm antibody, anti-Col2a-1 antibody, anti-Del1 antibody, anti-Lpk antibody, anti-Col2a antibody, anti-M100856 antibody, anti-Rgsc856 antibody, anti-ANFH antibody, anti-Col2 antibody, anti-BOS_5148 antibody, anti-SEDC antibody, anti-CG2A1A antibody, anti-COLLII antibody, anti-AOM antibody, anti-Collagen II antibody, anti-collagen II antibody
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IHC-P staining of pig liver tissue using Collagen II antibody (dilution at 1:300)
IF analysis of rabbit meniscus tissue using Collagen II antibody (dilution of primary antibody at 1:300)
Immunohistochemical analysis of formalin fixed and paraffin embedded rat intervertebral disc using Collagen II antibody (primary antibody at 1:300)
Immunohistochemical analysis of paraffin-embedded collagen of rat knee joint using Collagen II antibody (dilution of primary antibody - 1:300)
IHC-P of rat knee joint collagen (Collagen II antibody at 1:200)
Western blot analysis of human breast cancer (Lane 1), breast cancer (Lane 2) using Collagen II antibody (primary antibody at 1:200)
IHC-P of human esophageal carcinoma (Collagen II antibody atb 1:300)
Immunohistochemical staining of paraffin embedded human thyroid carcinoma tissue using Collagen II antibody (primary antibody at 1:300)
WB analysis of human breast cancer (Lane 1), breast cancer (Lane 2) using Collagen II antibody (dilution of primary antibody - 1:100)
Immunofluorescence analysis of mouse brain tissue using anti-Collagen II (dilution of primary antibody - 1:300)
IF image of mouse lung tissue using anti-Collagen II (primary antibody at 1:300)
IF analysis of mouse lung tissue using Collagen II antibody (dilution of primary antibody at 1:300)
Immunohistochemical staining of mouse lung tissue using anti-Collagen II (dilution of primary antibody - 1:300)
Immunofluorescence image of mouse brain tissue using anti-Collagen II (dilution at 1:300)
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IF
Immunofluorescence
ICC
Immunocytochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Chih-Hao Chen 1, Chia-Jui Chang 2, Chang-Yi Kuo 2, Guan-Jie Luo 3, Banendu Sunder Dash 2, Darshan Tagadur Govindaraju 2, Jyh-Ping Chen Chondroitin sulfate/hyaluronic acid/carboxymethylcellulose macroporous cryogels for controlled delivery of TGF-β1 and IGF-1 to induce chondrogenic differentiation of adipose-derived stem cells in cartilage tissue engineering Int J Biol Macromol, (2025)
Kuo, Chang-Yi et al. Incorporation of chitosan in biomimetic gelatin/chondroitin-6-sulfate/hyaluronan cryogel for cartilage tissue engineering Carbohydr Polym, 117, 722-730 (2015)
COL2A1 antibody (orb10436)
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