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COL6A5 Rabbit Polyclonal Antibody

SKU: orb2936
FeaturedFeatured Product

Description

COL6A5 Rabbit Polyclonal Antibody

Images & Validation

Tested ApplicationsIF, IHC-Fr, IHC-P
Dilution rangeIHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
ReactivityRat
Predicted ReactivityHuman

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human COL6A5/Collagen VI alpha 5 (121-220/2615aa)
TargetCOL6A5
Molecular Weight288 kDa
PurificationAffinity purified by Protein A
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
DisclaimerFor research use only

Alternative Names

Collagen VI alpha 5; CO6A5_HUMAN; COL29A1; Col6a5; Collagen alpha-1(XXIX) chain; Collagen alpha-5(VI) chain; Collagen, type VI, alpha 5; Collagen, type XXIX, alpha-1; Gm7455; von Willebrand factor A domain-containing protein 4; VWA4.

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Quality Guarantee

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COL6A5 Rabbit Polyclonal Antibody

Tissue/Cell: rat skin tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-COL6A5/Collagen VI alpha 5 Polyclonal Antibody, Unconjugated (orb2936) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

UniProt Details

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Protocol Information

IHC-P
Immunohistochemistry Paraffin
View Protocol
IHC-Fr
Immunohistochemistry Frozen
View Protocol
IF
Immunofluorescence
View Protocol

COL6A5 Rabbit Polyclonal Antibody (orb2936)

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Available Sizes

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50 μl
$ 240.00
100 μl
$ 390.00
200 μl
$ 510.00
DispatchUsually dispatched within 5-10 working days
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