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Embark on a seamless journey from antigen preparation to antibody validation with our Rabbit Monoclonal Antibody (mAb) Development Service. Whether starting from antigen preparation or leveraging our pre-validated B cell library, Biorbyt offers unparalleled expertise in generating high-quality rabbit monoclonal antibodies.
Our proprietary Single B platform unlocks the potential to produce over 10,000 rabbit monoclonal antibodies from just one immunized rabbit, yielding remarkable diversity. With over 5,000 pre-validated IgG sequences targeting 500+ drug targets, our proven approach ensures efficiency and effectiveness. Each target is accompanied by a corresponding B cell library, providing a shortcut for antibody screening and discovery.
Service Highlights
Efficient Single B Platform
Utilizing our proprietary single B platform, we can efficiently isolate and culture B cells from immunized rabbits.
Pre-validated B Cell Libraries
Find the right antibodies directly from the Biorbyt B cell libraries which reduces the risk and time involved in your research and development endeavors.
Optimization with Mammalian Cell Display
Optimized the mAb using our mammalian cell display-based antibody engineering system for the enhancement of binding affinity, stability, and other key properties.
Development Workflow
A breakdown of our end-to-end development phases:
Stage | Service Details | Deliverables | Time |
|---|---|---|---|
Immunogen preparation | Plasmid construction, mammalian cell produced immunogens | 7~10 weeks | |
Animal immunization | Immunize at least two rabbits against the same antigen | ELISA data on crude sera titer test | 5-8 Weeks |
B cell isolation & amplification | PBMC collection and cryopreservation; Positive B cell enrichment and single B cell expansion | ELISA test data on B cell culture supernatant | 2-3 Weeks |
IgG gene cloning & preliminary validation | IgG gene cloning, small-scale antibody production and validation; select 1~2 positive clones for full-length IgG sequencing. | Sequencing report of one clone with verified ELISA data | 3-4 Weeks |
mAb production and validation | Antibody production, purification and QC validation | 1mg purified recombinant antibody with QC data | 3-4 Weeks |
Please reach out to us at info@biorbyt.com to discuss your request for . Our scientists are here to answer your questions and provide you with the information and expertise you need for your projects.