CXCL9 Rabbit Polyclonal Antibody

SKU: orb783380

Featured

Description

Images & Validation

−

Item 1 of 3

Tested Applications	ELISA, WB
Dilution range	WB=1:500-2000, ELISA=1:5000-10000
Reactivity	Mouse
Predicted Reactivity	Rat

Related Conjugates & Formulations

−

Biotin

HRP

IRDye800

Key Properties

−

Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human CXCL9 (23-125/125aa)
Target	CXCL9
Molecular Weight	14 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

−

Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

−

MIG; CMK; C-X-C motif chemokine 9; CXCL9; chemokine(C-X-C motif) ligand 9; crg-10; Humig; monokine induced by gamma interferon; SCYB9; Small inducible cytokine B9; small inducible cytokine subfamily B member 9.

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CXCL9) Polyclonal Antibody, Unconjugated (orb783380) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Recombinant mouse CXCL9 protein, Primary: Anti-CXCL9 (orb783380) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 14 kDa, Observed band size: 20 kDa.

Tissue/Cell: human spleen tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-CXCL9/MIG Polyclonal Antibody, Unconjugated (orb783380) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

CXCL9

UniProt

Q07325

UniProt Details

−

No UniProt data available

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

Western Blot (IB, immunoblot)

View Protocol

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

View Protocol

Find More Protocols