CXCR3 Rabbit Polyclonal Antibody

SKU: orb10696

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Description

Research Area

CXC Chemokines, Chemokines, G Protein-Coupled Receptors, GPCR Signaling Pathway, Immunology, Innate Immunity, Signal Transduction

Images & Validation

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Item 1 of 3

Tested Applications	WB
Dilution range	WB=1:500-2000
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Bovine, Canine, Guinea pig, Porcine, Rabbit

Related Conjugates & Formulations

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Biotin

HRP

IRDye800

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human CXCR3 (1-100/415aa)
Target	CXCR3
Molecular Weight	41 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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CXCR3_HUMAN; Interferon-inducible protein 10 receptor; IP-10 receptor; C-X-C chemokine receptor type 3; CD 183; CD183; CD183 antigen; G protein-coupled receptor 9; G Protein Coupled Receptor 9; Chemokine(C X C motif) receptor 3; Chemokine(C X C) receptor 3; C-X-C Chemokine receptor; CXC Motif Receptor 3; Chemokine CXC Motif Receptor 3; CKRL2; CKR L2; CKR-L2; GPR9; CXC-R3; CXCR-3; IP10 receptor.

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Blank control: U937 (blue). Primary Antibody: Rabbit Anti-CXCR3 antibody (orb10696), Dilution: 1 ug in 100 uL 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol, The cells were fixed with 2% paraformaldehyde (10 min). Primary antibody (orb10696, 1 ug /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (Human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CXCR3) Polyclonal Antibody, Unconjugated (orb10696) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

Sample: Lane 1: Mouse Blood cell tissue lysates, Lane 2: Rat Blood cell tissue lysates, Primary: Anti-CXCR3 (orb10696) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 40 kDa, Observed band size: 40 kDa.

Quick Database Links

Gene Symbol

CXCR3

UniProt

P49682

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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