CYP11A1 Rabbit Polyclonal Antibody

SKU: orb5936

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Description

Research Area

Cell Biology, Cholesterol Metabolism, Hormone Biosynthesis, Hormone Research, Lipid Metabolism, Lipids and Lipoproteins Metabolism, Metabolism

Images & Validation

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Item 1 of 5

Tested Applications	WB
Dilution range	WB=1:500-5000
Reactivity	Rat
Predicted Reactivity	Mouse, Rat

Related Conjugates & Formulations

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Biotin

HRP

IRDye800

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human CYP11A1/P450SCC (321-420/521aa)
Target	CYP11A1
Molecular Weight	53 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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Cholesterol 20 22 desmolase; Cholesterol desmolase; Cholesterol monooxygenase(side chain cleaving); Cholesterol side chain cleavage enzyme; Cholesterol side chain cleavage enzyme mitochondrial; Cholesterol side-chain cleavage enzyme; CP11A_HUMAN; CYP11A1; CYPXIA1; Cytochrome P450 11A1; Cytochrome P450 11A1 mitochondrial; Cytochrome P450 family 11 subfamily A polypeptide 1; Cytochrome P450 subfamily XIA; Cytochrome P450(scc); Cytochrome P450C11A1; mitochondrial; P450SCC; Steroid 20 22 lyase.

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Sample: Placenta (Mouse) Lysate at 30 ug, Primary: Anti-CYP11A1 (Ser576) (orb5936) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution, Predicted band size: 53/57 kD, Observed band size: 57 kD.

Paraformaldehyde-fixed, paraffin embedded (mouse adrenal gland), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CYP11A1) Polyclonal Antibody, Unconjugated (orb5936) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat adrenal gland tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CYP11A1) Polyclonal Antibody, Unconjugated (orb5936) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat adrenal gland), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CYP11A1) Polyclonal Antibody, Unconjugated (orb5936) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Brain (Rat) Lysate at 30 ug, Lane 2: Kidney (Rat) Lysate at 30 ug, Primary: Anti-CYP11A1/P450SCC (orb5936) at 1:200 dilution, Secondary: HRP conjugated Goat Anti-Rabbit IgG (orb572747) at 1:3000 dilution, Predicted band size: 53kD, Observed band size: 53kD.

Quick Database Links

Gene Symbol

CYP11A1

UniProt

P05108

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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