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Dextranase (Penicillium Species) Antibody Biotin Conjugated
SKU: orb344752
Description
Images & Validation
−Item 1 of 2
| Tested Applications | ELISA, IP, WB |
|---|---|
| Dilution range | ELISA: 1:2,000 - 1:10,000, IP: 1:100, WB: 1:500 - 1:5,000 |
| Reactivity | Algae |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Dextranase [Penicillium species] DEX |
| Purity | Anti-Dextranase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Dextranase [Penicillium species]. Cross reactivity against Dextranase from other sources is unknown. |
| Conjugation | Biotin |
Storage & Handling
−| Storage | Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing. |
|---|---|
| Form/Appearance | Liquid (sterile filtered) |
| Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
| Concentration | 1.0 mg/mL |
| Dry Ice Shipping | Please note: This product requires shipment on dry ice. A dry ice surcharge will apply. |
| Disclaimer | For research use only |
Alternative Names
−rabbit anti-Dextranase Antibody biotin Conjugation, biotin Conjugated rabbit anti-Dextranase Antibody, dextranase, Alpha-1,6-glucan-6-glucanohydrolase, DEX
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1 ml- Item 1 of 2
Dextranase (Penicillium Species) Antibody Biotin Conjugated [orb344751]
ELISA, IP, WB
Algae
Rabbit
Polyclonal
Biotin
100 μg
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ELISA Results of Rabbit Anti-Dextranase Antibody Biotin Conjugated tested against purified Dextranase protein. Each well was coated in duplicate with 1.0 µg of Dextranase. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Stabilizer, Streptavidin-HRP conjugated (p/n orb348767), and TMB substrate (p/n orb348651).
Western Blot of Anti-Dextranase Antibody Biotin Conjugated. Lane 1: Molecular Weight Marker. Lane 2: Dextranase. Primary Antibody: Rabbit Anti-Dextranase Biotin Conjugate at 1:1000 overnight at 2-8°C. Secondary Antibody: Streptavidin-HRP (p/n orb348767) at 1:40000 for 30 min at RT. Block: BlockOut Buffer (p/n orb348644). Expected MW: ~66kDa.
Quick Database Links
UniProt
RefSeq:AAB47720.1
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| RefSeq | AAB47720.1 |
|---|
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
IP
Immunoprecipitation
Dextranase (Penicillium Species) Antibody Biotin Conjugated (orb344752)
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