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Dextranase (Penicillium Species) Antibody Biotin Conjugated
SKU: orb344751
Description
Images & Validation
−Item 1 of 2
| Tested Applications | ELISA, IP, WB |
|---|---|
| Dilution range | ELISA: 1:2,000 - 1:10,000, IP: 1:100, WB: 1:500 - 1:5,000 |
| Reactivity | Algae |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Dextranase [Penicillium species] DEX |
| Purity | Anti-Dextranase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Dextranase [Penicillium species]. Cross reactivity against Dextranase from other sources is unknown. |
| Conjugation | Biotin |
Storage & Handling
−| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
| Concentration | 1.0 mg/mL |
| Disclaimer | For research use only |
Alternative Names
−rabbit anti-Dextranase Antibody biotin Conjugation, biotin Conjugated rabbit anti-Dextranase Antibody, dextranase, Alpha-1,6-glucan-6-glucanohydrolase, DEX
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1 ml- Item 1 of 2
Dextranase (Penicillium Species) Antibody Biotin Conjugated [orb344752]
ELISA, IP, WB
Algae
Rabbit
Polyclonal
Biotin
25 μl
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ELISA Results of Rabbit Anti-Dextranase Antibody Biotin Conjugated tested against purified Dextranase protein. Each well was coated in duplicate with 1.0 µg of Dextranase. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Stabilizer, Streptavidin-HRP conjugated (p/n orb348767), and TMB substrate (p/n orb348651).
Western Blot of Anti-Dextranase Antibody Biotin Conjugated. Lane 1: Molecular Weight Marker. Lane 2: Dextranase. Primary Antibody: Rabbit Anti-Dextranase Biotin Conjugate at 1:1000 overnight at 2-8°C. Secondary Antibody: Streptavidin-HRP (p/n orb348767) at 1:40000 for 30 min at RT. Block: BlockOut Buffer (p/n orb348644). Expected MW: ~66kDa.
Quick Database Links
UniProt
RefSeq:AAB47720.1
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| RefSeq | AAB47720.1 |
|---|
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
IP
Immunoprecipitation
Dextranase (Penicillium Species) Antibody Biotin Conjugated (orb344751)
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