ECFP Mouse Monoclonal Antibody

SKU: orb500318

Description

Images & Validation

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Item 1 of 1

Tested Applications	ELISA, WB
Dilution range	WB=1:1000-5000, ELISA=1:1000-5000
Reactivity	All

Related Conjugates & Formulations

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Biotin

HRP

IRDye800

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	IgG
Clone No.	10H5
Immunogen	Recombinant ECFP
Purification	Affinity purified by Protein G
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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Cyan Fluorescent Protein; Enhanced Cyan Fluorescent Protein.

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Sample: Lane 1: ECFP-Tagged fusion protein (full length) Overexpression E.coli Lysate at 4 ug, Primary: Anti-ECFP-Tag (orb500318) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 27 kD, Observed band size: 27 kD.

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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