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EGF Receptor / EGFR Antibody
SKU: orb749350
Description
Images & Validation
−Item 1 of 3
| Tested Applications | FACS, IF |
|---|---|
| Dilution range | Flow cytometry: 5ul per test per one million cells in 0.1ml or 5ul per 100ul of whole blood,Immunofluorescence: 1:50-1:100 |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG2a, kappa |
| Clone No. | GFR450 |
| Immunogen | Recombinant extracellular domain of human EGFR protein was used as the immunogen for the EGFR antibody. |
| Conjugation | CF488 |
Storage & Handling
−| Storage | Store the EGFR antibody at 2-8°C, protected from light. |
|---|---|
| Disclaimer | For research use only |
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EGF Receptor / EGFR Antibody [orb749487]
FACS, IF, IHC-P
Human
Mouse
Monoclonal
Unconjugated
20 μg, 100 μg - Item 1 of 3
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Left: Immunofluorescent staining of human A431 cells with CF488-conjugated EGFR antibody (clone GFR450, green) and DAPI nuclear stain (blue). Right: isotype control.
Flow cytometry testing of human A431 cells with EGFR antibody (clone GFR450); Black = cells alone, Gray = isotype control, Green = EGFR antibody.
Flow cytometry testing of mouse NIH3T3 cells with EGFR antibody (clone GFR450); Black = cells alone, Gray = isotype control, Green = EGFR antibody. This antibody is not suitable for mouse sample testing.
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Protocol Information
FACS
Fluorescence-Activated Cell Sorting (FC, Flow cytometry)
IF
Immunofluorescence
EGF Receptor / EGFR Antibody (orb749350)
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