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EGFR Antibody
SKU: orb749349
Description
Images & Validation
−Item 1 of 4
| Tested Applications | FACS, IF |
|---|---|
| Dilution range | Flow cytometry: 1-2ug/10^6 cells,Immunofluorescence: 1-2ug/ml |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG2a, kappa |
| Clone No. | GFR450 |
| Immunogen | The immunizing protein for this antibody was recombinant human EGFR. |
| Purification | Protein G affinity chromatography |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | 1X PBS, pH 7.4 |
| Disclaimer | For research use only |
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FACS testing of A431 cells with isotype control (gray), without primary antibody (black) and EGFR antibody (green, clone GFR450).
Immunofluorescent staining of A431 cells with Alexa Fluor conjugated EGFR antibody (green, clone GFR450) and DAPI nuclear stain (blue).
Analysis of HuProt (TM) microarray containing more than 19, 000 full-length human proteins using EGFR antibody (clone GFR450). These results demonstrate the foremost specificity of the GFR450 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
SDS-PAGE analysis of purified, BSA-free EGFR antibody (clone GFR450) as confirmation of integrity and purity.
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Protocol Information
FACS
Fluorescence-Activated Cell Sorting (FC, Flow cytometry)
IF
Immunofluorescence
EGFR Antibody (orb749349)
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