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EGFR5 Rabbit Polyclonal Antibody
SKU: orb1971
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Description
Images & Validation
−Item 1 of 2
| Tested Applications | FC, WB |
|---|---|
| Dilution range | WB=1:500-2000, Flow-Cyt=1μg/Test |
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from human EGFR5/CLEC14A (221-320/490aa) |
| Target | CLEC14A |
| Molecular Weight | 49 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Disclaimer | For research use only |
Alternative Names
−C type lectin domain family 14, member A; C14orf27; CEG1; ClECT and EGF like domain containing protein; EGFR5; EGFR-5; EGFR 5; Epidermal growth factor receptor 5; CLC14_HUMAN.
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Blank control (blue): 293T (fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti-EGFR5 antibody (orb1971), dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), dilution: 1:200 in 1X PBS containing 0.5% BSA.
Sample: B16 Cell (Mouse) Lysate at 40 ug, DU145 Cell (Human) Lysate at 40 ug, Primary: Anti-EGFR5 (orb1971) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 49 kD, Observed band size: 53 kD.
Quick Database Links
Gene Symbol
CLEC14A
UniProt
UniProt Details
− No UniProt data available
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
FC
Flow Cytometry
EGFR5 Rabbit Polyclonal Antibody (orb1971)
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