You have no items in your shopping cart.
ENO1 Rabbit Polyclonal Antibody
SKU: orb5156
Featured
Description
Research Area
Cancer Research, Cell Biology, DNA and RNA Research, Energy Metabolism, Metabolism, Transcription Factors, Tumor Suppressors
Images & Validation
−Item 1 of 2
| Tested Applications | IF, IHC-Fr, IHC-P, WB |
|---|---|
| Dilution range | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Bovine, Rabbit |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from human ENO1 (51-150/434aa) |
| Target | ENO1 |
| Molecular Weight | 47 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Disclaimer | For research use only |
Alternative Names
−2 phospho D glycerate hydro lyase; alpha enolase 1; alpha enolase; C myc promoter binding protein; EC 4.2.1.11; ENO1L1; Enolase 1(alpha); Enolase 1(alpha) like 1; Enolase 1; MBP 1; MBP1; MBPB1; MPB 1; MPB1; MYC promoter binding protein 1; NNE; Non neural enolase; phosphopyruvate hydratase; Plasminogen binding protein; PPH; Non Neuronal Enolase; ENOA_HUMAN.
Similar Products
−- Item 1 of 6
ENO1 Rabbit Polyclonal Antibody [orb575056]
IHC, WB
Bovine, Canine, Equine, Guinea pig, Mouse, Porcine, Rabbit, Rat, Zebrafish
Human
Rabbit
Polyclonal
Unconjugated
100 μl - Item 1 of 4
ENOA Antibody (C-term) [orb1931370]
FC, IF, IHC-P, WB
Human, Mouse
Rabbit
Polyclonal
Unconjugated
400 μl - Item 1 of 4
ENO1 antibody [N3C3] [orb555988]
DOT, ICC, IHC, IHC-P, WB
Human, Mouse, Rat, Zebrafish
Rabbit
Polyclonal
Unconjugated
100 μl - Item 1 of 4
- Item 1 of 3
ENO1 Rabbit Polyclonal Antibody [orb575055]
IHC, WB
Bovine, Canine, Equine, Guinea pig, Mouse, Rabbit, Sheep, Yeast, Zebrafish
Human, Rat
Rabbit
Polyclonal
Unconjugated
100 μl
![ENO1 antibody [N3C3]](/images/pub/media/catalog/product/e/n/eno1-antibody_orb555988_ifa_1.jpg)
![ENO1 antibody [N3C3]](/images/pub/media/catalog/product/e/n/eno1-antibody_orb555988_ihc_1.jpg)
![ENO1 antibody [N3C3]](/images/pub/media/catalog/product/e/n/eno1-antibody_orb555988_wb_1.jpg)
![ENO1 antibody [N3C3]](/images/pub/media/catalog/product/e/n/eno1-antibody_orb555988_wb_2.jpg)
Quality Guarantee
Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.
25 ug total protein per lane of various lysates probed with ENO1 polyclonal antibody, unconjugated (orb5156) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Tissue/Cell: Rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-ENO1 Polyclonal Antibody, Unconjugated (orb5156) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated (orb868589) used at 1:200 dilution for 40 minutes at 37°C. DAPI (5 ug/ml, blue) was used to stain the cell nuclei.
Quick Database Links
Gene Symbol
ENO1
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
Request a Document
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
IF
Immunofluorescence
ENO1 Rabbit Polyclonal Antibody (orb5156)
- 0.0
Based on 0 reviews
Participating in our Biorbyt product reviews program enables you to support fellow scientists by sharing your firsthand experience with our products.
Login to Submit a ReviewAvailable Sizes
Select a size below
Usually dispatched within 5-10 working days