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Fibronectin/FN1 Rabbit Polyclonal Antibody (BF647)
Description
Images & Validation
−| Tested Applications | IF |
|---|---|
| Dilution range | IF=1:200-800 |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Bovine, Canine |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from human Fibronectin/Ugl-Y3 (1201-1300/2386aa) |
| Target | FN1 |
| Purification | Affinity purified by Protein A |
| Conjugation | BF647 |
Storage & Handling
−| Storage | Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
|---|---|
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Disclaimer | For research use only |
Alternative Names
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Paraformaldehyde-fixed, paraffin embedded (Rat colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation: Anti-Fibronectin Antibody, conjugated (orb1577419) 1:400, 1.5 hours at 37°C, DAPI (5 ug/ml, blue) was used to stain the cell nuclei.
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Fibronectin/FN1 Rabbit Polyclonal Antibody (BF647) (orb1577419)
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