FLIP Rabbit Polyclonal Antibody

SKU: orb10664

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Description

Images & Validation

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Tested Applications	IF, IHC-Fr, IHC-P, WB
Dilution range	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Bovine, Canine, Equine, Gallus, Porcine, Rabbit

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human CASP8 and FADD-like apoptosis regulator subunit p12 (Potential) (401-480/480aa)
Target	CFLAR
Molecular Weight	12/52 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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CASP8 and FADD-like apoptosis regulator subunit p12; c FLIP; c FLIPL; c FLIPR; c FLIPS; CASH; CASP8 and FADD like regulator; CASP8 and FADD like apoptosis regulator precursor; CASP8AP1; Caspase Eight Related Protein; Caspase homolog; Caspase Homologue; Caspase Like Apoptosis Regulatory Protein; Caspase related inducer of apoptosis; CASPER; Cellular FLICE like inhibitory protein; CFLA; CFLAR; CLARP; FADD like anti apoptotic molecule; FADD Like Anti-apoptotic Molecule 1; FADD like antiapoptotic molecule 1; FADD Like Apoptosis Regulator; FLAME 1; FLAME; FLAME1; FLICE Inhibitor Protein; FLIP; FLIPs; I FLICE; Inhibitor of FLICE; MACH Related Inducer of Toxicity; mFLIP; MRIT; USURPIN; Usurpin beta; FLICE-like inhibitory protein long form.

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Sample: Heart (Mouse) Cell Lysate at 40 ug, Primary: Anti-FLIP (orb10664) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 12/52 kD, Observed band size: 52 kD.

Sample: Heart (Mouse) Lysate at 40 ug, Primary: Anti-FLIP (orb10664) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 12/52 kD, Observed band size: 52 kD.

Quick Database Links

Gene Symbol

CFLAR

UniProt

O15519

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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Find More Protocols

Grzesiak, Malgorzata et al. Effect of dietary supplementation with nettle or fenugreek on folliculogenesis and steroidogenesis in the rabbit ovary - An in vivo study Theriogenology, 173, (2021)

PubMed: 34126406