Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from mouse GM-CSFR alpha (101-230/388aa)
Target	CSF2RA
Molecular Weight	38 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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Granulocyte-macrophage colony-stimulating factor receptor subunit alpha; GM-CSF Receptor alpha; CD116; CD116 antigen; CDw116; Colony Stimulating Factor 2 Receptor alpha; Colony stimulating factor 2 receptor alpha chain; CSF 2R; CSF2RA; CSF2RAX; CSF2RAY; CSF2RX; CSF2RY; GM CSF R alpha; GM CSF receptor alpha subunit; GMCSFR; GMR; Granulocyte Macrophage Colony Stimulating Factor Receptor alpha Chain; GM-CSF-R-alpha; GM-CSF R alpha; GMCSFR-alpha; GMR-alpha; CSF2R_MOUSE; Granulocyte-macrophage colony-stimulating factor receptor subunit alpha; CSF2R_HUMAN.

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GM-CSFR alpha Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Human Melanocytoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (GM-CSFR alpha) Polyclonal Antibody, Unconjugated (orb10732) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Tissue/Cell: human colon carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-GM-CSFR alpha/CD116 Polyclonal Antibody, Unconjugated (orb10732) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/Cell: mouse intestine tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-GM-CSFR alpha/CD116 Polyclonal Antibody, Unconjugated (orb10732) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated (orb868589) used at 1:200 dilution for 40 minutes at 37°C. DAPI (5 ug/ml, blue) was used to stain the cell nuclei.

Quick Database Links

Gene Symbol

CSF2RA

UniProt

Q00941

UniProt Details

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No UniProt data available

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Protocol Information

IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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