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Goat Anti-Mouse IgG H&L, FITC conjugated
Description
Images & Validation
−| Tested Applications | FC, ICC, IF |
|---|---|
| Dilution range | IF=1:200-1000, Flow-Cyt=1:50-200, ICC/IF=1:100-1000 |
| Reactivity | Mouse |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Secondary Antibody |
|---|---|
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Native Mouse IgG |
| Purification | Affinity purified by Protein G, nonspecific adsorbed |
| Conjugation | FITC |
Storage & Handling
−| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
|---|---|
| Buffer/Preservatives | 10 mM TBS (pH=7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% glycerol. |
| Concentration | 2.0 mg/ml |
| Disclaimer | For research use only |
Alternative Names
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Paraformaldehyde-fixed, paraffin embedded (Human colon cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Cytokeratin 8) Monoclonal Antibody, Unconjugated (orb500822) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Mouse IgG antibody (orb868188) for 90 minutes, and DAPI for nuclei staining.
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Goat Anti-Mouse IgG H&L, FITC conjugated (orb868188)
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