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Description
Research Area
Cell Biology
Images & Validation
−
Item 1 of 3
| Tested Applications | IF, IHC-Fr, IHC-P, WB |
|---|---|
| Dilution Range | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
| Reactivity | Human, Rat |
| Predicted Reactivity | Mouse, Porcine, Rabbit |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from human GRP78 (251-360/654aa) |
| Target | HSPA5 |
| Molecular Weight | 74 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−BIP; GRP78; HEL-S-89n; BIP_HUMAN; HSPA5; 78 kDa glucose-regulated protein (GRP-78); Binding-immunoglobulin protein (BiP); Heat shock protein 70 family protein 5 (HSP70 family protein 5); Heat shock protein family A member 5; Immunoglobulin heavy chain-binding protein; 3.6.4.10; heat shock protein family A (Hsp70) member 5; heat shock 70kD protein 5 (glucose-regulated protein, 78kD); heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa); glucose-regulated protein, 78kDa
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Quick Database Links
Gene Symbol
HSPA5
UniProt
UniProt Details
− No UniProt data available
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
IF
Immunofluorescence
Xia, Dan et al. Knockout of MARCH2 inhibits the growth of HCT116 colon cancer cells by inducing endoplasmic reticulum stress Cell Death Dis, 8, e2957 (2017)
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