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HA Tag Mouse Monoclonal Antibody
SKU: orb500021
Description
Images & Validation
−Item 1 of 3
| Tested Applications | ELISA, WB |
|---|---|
| Dilution range | WB=1:10000-100000, ELISA=1:1000-5000 |
| Reactivity | All |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone No. | 4D1 |
| Immunogen | KLH conjugated HA Tag |
| Purification | Affinity purified by Protein G |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Disclaimer | For research use only |
Alternative Names
−HA-tag; HA epitope tag; HA1; HA2; hemagglutinin; Hemagglutinin HA1 chain; Hemagglutinin HA2.
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Sample: HA-Tagged Fusion Protein Overexpression E.coli Lysate (orb1516792) at 4 ug, Primary: Anti-HA-Tag (orb500021) at 1/1000 ~ 1/10000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 41 kD, Observed band size: 41 kD.
Sample: HA-Tagged fusion protein, Primary: Anti-HA-tag/HRP at 1/100000 dilution, Observed band size: 42 kD.
Sample: Lane 1: HA-Tagged Fusion Protein Overexpression E.coli Lysate (orb1516792) at 2 ug Lane 2: HA-Tagged Fusion Protein Overexpression E.coli Lysate (orb1516792) at 2 ug Primary: Anti-HA-Tag (orb500021) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 41 kD Observed band size: 45 kD.
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Protocol Information
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
HA Tag Mouse Monoclonal Antibody (orb500021)
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