HAVCR2/TIM-3 Rabbit Polyclonal Antibody

SKU: orb186446

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Description

Research Area

Immunology & Inflammation

Images & Validation

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Item 1 of 4

Tested Applications	ELISA, FC, ICC, WB
Dilution Range	WB=1:500-2000, Flow-Cyt=1ug/Test, ELISA=1:5000-10000, ICC/IF=1:50-200
Reactivity	Human, Mouse
Predicted Reactivity	Human

Related Conjugates & Formulations

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Biotin

HRP

IRDye800

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Recombinant human TIM-3 protein (22-200/301aa)
Target	HAVCR2
Molecular Weight	29 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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CD366; HAVcr-2; KIM-3; SPTCL; TIM3; TIMD-3; TIMD3; Tim-3; HAVR2_HUMAN; HAVCR2; T-cell immunoglobulin and mucin domain-containing protein 3 (TIMD-3); T-cell immunoglobulin mucin receptor 3 (TIM-3); T-cell membrane protein 3;

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Sample: Liver (Rat) Lysate at 40 ug, Primary: Anti-TIM 3 (orb186446) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 29 kD, Observed band size: 49 kD.

Sample: Spleen (Mouse) Lysate at 40 ug, Primary: Anti-TIM 3 (orb186446) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 29 kD, Observed band size: 49 kD.

Sample: Thymus (Mouse) Lysate at 40 ug, Raw264.7 (Mouse) Cell Lysate at 30 ug, Primary: Anti-TIM 3 (orb186446) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 29 kD, Observed band size: 49 kD.

Tissue/Cell: rat liver tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-TIM-3 Polyclonal Antibody, Unconjugated (orb186446) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

HAVCR2

UniProt

Q8TDQ0

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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Flow Cytometry

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ICC

Immunocytochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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