Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Recombinant human HES1 protein (1-277/280aa)
Target	HES1
Molecular Weight	30 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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bHLHb39; C-HAIRY1; c-hairy1A; Class B basic helix-loop-helix protein 39; FLJ20408; Hairy and enhancer of split 1(Drosophila); Hairy and enhancer of split 1; Hairy Enhancer of Split 1; Hairy homolog(Drosophila); Hairy Homolog; Hairy like; Hairy, Drosophila, homolog of; Hairy-like protein; Hairy/enhancer of split, Drosophila, homolog of, 1; HAIRY1; HES-1; hes1; Hes1 hairy and enhancer of split 1(Drosophila); HES1_HUMAN; HHL; HL; HRY; RHL; Transcription factor HES 1; Transcription factor HES-1.

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Measured by its binding ability in a indirect ELISA. Immobilized Human HES1 protein, His Tag at 2 µg/mL (100 µl/well) can bind Rabbit Anti-Human HES1 Antibody, the EC50 is 178.8 ng/ml.

Sample: Lane 1: Rat Kidney tissue lysates, Lane 2: Rat Liver tissue lysates, Primary: Anti-HES1 (orb1602466) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 30 kDa, Observed band size: 35 kDa.

Sample: Lane 1: Recombinant human HES1 protein, Primary: Anti-HES1 (orb1602466) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 30 kDa, Observed band size: 30 kDa.

Quick Database Links

Gene Symbol

HES1

UniProt

Q14469

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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ICC

Immunocytochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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