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His tag Rabbit Polyclonal Antibody

SKU: orb10813

Description

His tag Rabbit Polyclonal Antibody

Research Area

6x his tag, His Tag

Images & Validation

Tested ApplicationsELISA, FC, ICC, IF, IHC-Fr, IHC-P, WB
Dilution rangeWB=1:500-2000, ELISA=1:1000-5000, IHC-P=1:200-1000, IHC-F=1:200-1000, IF=1:200-1000, Flow-Cyt=1ug/Test, ICC/IF=1:100-500
ReactivityAll

Related Conjugates & Formulations

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenSynthetic peptide: HHHHHH conjugated to KLH
PurificationAffinity purified by Protein A
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
DisclaimerFor research use only

Alternative Names

HisX6; 6His; His-Tag; 6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; Histidine.; His Tag;

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His tag Rabbit Polyclonal Antibody

Sample: His-Tagged Fusion Protein Overexpression E.coli Lysate at 4 ug, Primary: Anti-His Tag (orb10813) at 1/2000 ~ 1/5000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 51 kD, Observed band size: 51 kD.

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC-P
Immunohistochemistry Paraffin
View Protocol
IHC-Fr
Immunohistochemistry Frozen
View Protocol
FC
Flow Cytometry
View Protocol
IF
Immunofluorescence
View Protocol
ICC
Immunocytochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

His tag Rabbit Polyclonal Antibody (orb10813)

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Available Sizes

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100 μl
$ 220.00
200 μl
$ 220.00
500 μl
$ 630.00
1 ml
$ 670.00
DispatchUsually dispatched within 5-10 working days
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