Histone H4 (Acetyl K5) Rabbit Polyclonal Antibody

SKU: orb312267

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Description

Images & Validation

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Tested Applications	ICC, IF, IHC-Fr, IHC-P, WB
Dilution range	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500
Reactivity	Human, Rat
Predicted Reactivity	Bovine, Canine, Equine, Mouse, Porcine, Rabbit

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthesised acetylpeptide derived from human Histone H4 around the acetylation site of Acetyl Lys5 RG(Ac-K)GG
Target	H4C1
Molecular Weight	11 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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Histone H4(Acetyl K5); Histone H4(Acetyl Lys5); Acetyl-Histone H4(K5); Acetyl-Histone H4(Lys5); H4F2; H4FN; HIST1H4; HIST2H4; HISTH4H4; methyl histone H4; histone H4; H4_HUMAN; Osteogenic growth peptide; OGP.

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Histone H4 (Acetyl K5) Rabbit Polyclonal Antibody

Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (Histone H4 (Acetyl K5)) polyclonal Antibody, Unconjugated (orb312267) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Paraformaldehyde-fixed, paraffin embedded (rat liver tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (H4 (Acetyl K5)) Polyclonal Antibody, Unconjugated (orb312267) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Human Hela cell lysates, Lane 2: Human Jurkat cell lysates, Lane 3: Human HL60 cell lysates, Lane 4: Human MCF-7 cell lysates, Primary: Anti-Histone H4 (Acetyl K5) (orb312267) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 11 kD, Observed band size: 11 kD.

Quick Database Links

Gene Symbol

H4C1

UniProt

P62805

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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ICC

Immunocytochemistry

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