Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from HPV16 E7 protein (11-70/98aa)
Target	L2
Molecular Weight	11 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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E7; HPV16 E7 protein; Human Papilloma Virus; Human papillomavirus type 16 E7; Human papillomavirus type 16 E7; Protein E7; Human Papillomavirus 16(E7); HPV16 E7; E7 protein(HPV16); VE7_HPV16.

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Blank control (black line): Hela. Primary Antibody (green line): Rabbit Anti-HPV16 E7 antibody (orb159194), dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488, dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: hela (Human) Cell Lysate at 30 ug, mcf-7 (Human) Cell Lysate at 30 ug, mda-mb-231 (Human) Cell Lysate at 30 ug, siha (Human) Cell Lysate at 30 ug, recombinant HPV16-E7 protein 100 ng, recombinant HPV16-E7 protein 50 ng, Primary: Anti-HPV16 E7 (orb159194) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution.

Sample: Lane 1: HPV16 E7 fusion protein 0.1 ug Lysates, Lane 2: HPV16 E7 fusion protein 0.05 ug Lysates, Primary: Anti-HPV16 E7 (orb159194) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 11kDa, Observed band size: 50kDa.

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