Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	IgG
Clone No.	4E5
Immunogen	Recombinant human ICAM1/CD54 (321-420/532aa)
Target	ICAM1
Molecular Weight	56 kDa
Purification	Affinity purified by Protein G
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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BB2; CD54 antigen; CD54; ICAM1; ICAM 1; ICAM-1; Cell surface glycoprotein P3.58; Human rhinovirus receptor; Intercellular adhesion molecule 1; Major group rhinovirus receptor; MALA2; MyD10; P3.58; Surface antigen of activated B cells; ICAM1_HUMAN; MALA-2; MyD10; intercellular adhesion molecule 1 precursor; intercellular adhesion molecule 1(CD54), human rhinovirus receptor; major group rhinovirus receptor.

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Paraformaldehyde-fixed, paraffin embedded (Human liver cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (ICAM1/CD54) Monoclonal Antibody, Unconjugated (orb500866) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Human lung cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (ICAM1/CD54) Monoclonal Antibody, Unconjugated (orb500866) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.

Sample: A549 (Human) Cell Lysate at 30 ug, MCF-7 (Human) Cell Lysate at 30 ug, Primary: Anti-ICAM1'CD54 (orb500866) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 56 kD, Observed band size: 90 kD.

Sample: HUVEC (Human) Cell Lysate at 30 ug, Primary: Anti-ICAM1/CD54 (orb500866) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 56 kD, Observed band size: 100 kD.

Quick Database Links

Gene Symbol

ICAM1

UniProt

P05362

UniProt Details

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No UniProt data available

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Protocol Information

IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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