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IGF2R/M6PR Rabbit Polyclonal Antibody

SKU: orb500521

Description

IGF2R/M6PR Rabbit Polyclonal Antibody

Research Area

Cell Biology, Endosomes, Inflammation, Macrophage, Signal Transduction

Images & Validation

Tested ApplicationsFC
Dilution rangeFlow-Cyt=1ug/Test
ReactivityHuman
Predicted ReactivityBovine, Mouse, Rat

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human IGF2R/M6PR (701-800/2491aa)
TargetIGF2R
Molecular Weight300 kDa
PurificationAffinity purified by Protein A
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
DisclaimerFor research use only

Alternative Names

300 kDa mannose 6-phosphate receptor; Cation independent mannose 6 phosphate receptor; Cation-independent mannose-6-phosphate receptor; CD222; CD222 antigen; CI Man 6 P receptor; CI Man-6-P receptor; CI MPR; CI-MPR; CIMPR; IGF 2 receptor; IGF 2R; IGF II receptor; IGF-II receptor; IGF2 receptor; IGF2R; Insulin like growth factor 2 receptor; Insulin like growth factor II receptor; Insulin-like growth factor 2 receptor; Insulin-like growth factor II receptor; M6P R; M6P/IGF2 receptor; M6P/IGF2R; M6PR; MPR 300; MPR300; MPRI; MPRI_HUMAN.

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IGF2R/M6PR Rabbit Polyclonal Antibody

Blank control (Black line): Molt4 (Black). Primary Antibody (green line): Rabbit Anti-IGF2R/M6PR antibody (orb500521), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 15 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

UniProt Details

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Protocol Information

FC
Flow Cytometry
View Protocol

IGF2R/M6PR Rabbit Polyclonal Antibody (orb500521)

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50 μl
$ 250.00
100 μl
$ 390.00
200 μl
$ 470.00
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