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IRS1 Rabbit Polyclonal Antibody
SKU: orb10936
Featured
Description
Research Area
Cancer Research, Cardiovascular Research, Cell Biology, Cytoplasmic, Diabetes Research, Insulin and Insulin Like, Metabolism, Obesity, Signal Transduction, Tumor Biology
Images & Validation
−Item 1 of 3
| Tested Applications | IF, IHC-Fr, IHC-P |
|---|---|
| Dilution range | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-300 |
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Bovine, Equine, Human, Porcine, Rabbit, Sheep |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from rat IRS-1 (1101-1200/1242aa) |
| Target | IRS1 |
| Molecular Weight | 137 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Disclaimer | For research use only |
Alternative Names
−IRS1_HUMAN; Insulin receptor substrate 1; IRS-1; IRS 1;
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Paraformaldehyde-fixed, paraffin embedded Mouse Cerebellum, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with IRS1 Polyclonal Antibody, Unconjugated (orb10936) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Pancreas, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with IRS1 Polyclonal Antibody, Unconjugated (orb10936) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with IRS1 Polyclonal Antibody, Unconjugated (orb10936) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Quick Database Links
Gene Symbol
IRS1
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
IF
Immunofluorescence
Ning, Chong et al. Chicory inulin ameliorates type 2 diabetes mellitus and suppresses JNK and MAPK pathways in vivo and in vitro Mol Nutr Food Res, 61, (2017)
IRS1 Rabbit Polyclonal Antibody (orb10936)
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