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JIP1/MAPK8IP1 Rabbit Polyclonal Antibody

SKU: orb101108
FeaturedFeatured Product

Description

JIP1/MAPK8IP1 Rabbit Polyclonal Antibody

Images & Validation

Tested ApplicationsIF, IHC-Fr, IHC-P, WB
Dilution RangeWB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
ReactivityHuman, Rat
Predicted ReactivityBovine, Canine, Equine, Mouse, Porcine, Sheep

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human JIP1/MAPK8IP1 (425-524/711aa)
TargetMAPK8IP1
Molecular Weight77 kDa
PurificationAffinity purified by Protein A
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
DisclaimerFor research use only

Alternative Names

C jun amino terminal kinase interacting protein 1; IB 1; IB1; Islet brain 1; JIP 1; JNK interacting protein 1; JNK MAP kinase scaffold protein 1; MAPK8IP 1; MAPK8IP1; Mitogen activated protein kinase 8 interacting protein 1; PRKM8 interacting protein; PRKM8IP; JIP1_HUMAN.

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JIP1/MAPK8IP1 Rabbit Polyclonal Antibody

Sample: HepG2 cell (human) Lysate at 40 ug, Primary: Anti-JIP1/MAPK8IP1 (orb101108) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 77 kD, Observed band size: 77 kD.

JIP1/MAPK8IP1 Rabbit Polyclonal Antibody

Tissue/cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-MAPK8IP1 Polyclonal Antibody, Unconjugated (orb101108) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

UniProt Details

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC-P
Immunohistochemistry Paraffin
View Protocol
IHC-Fr
Immunohistochemistry Frozen
View Protocol
IF
Immunofluorescence
View Protocol

JIP1/MAPK8IP1 Rabbit Polyclonal Antibody (orb101108)

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50 μl
$ 250.00
100 μl
$ 390.00
200 μl
$ 470.00
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