Ki-67 Rabbit Polyclonal Antibody

SKU: orb13523

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Description

Images & Validation

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Item 1 of 5

Tested Applications	FC, ICC, IF, IHC-Fr, IHC-P, WB
Dilution range	WB=1:500-2000, IHC-P=1:200-800, IHC-F=1:200-800, ICC/IF=1:100-500, IF=1:200-800, Flow-Cyt=1ug/Test
Reactivity	Human, Mouse, Rat

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human Ki67
Target	MKI67
Molecular Weight	320/359 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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Antigen identified by monoclonal antibody Ki 67; Antigen KI67; KIA; Ki67; MKI67; Proliferation related Ki 67 antigen; Antigen KI-67; KI67_HUMAN.

Quality Guarantee

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4% Paraformaldehyde-fixed Hela (H) cell, Triton X-100 at r.t. for 20 min, Antibody incubation with (Ki-67) polyclonal Antibody, unconjugated (orb13523) 1:100, 90 min at 37°C, followed by conjugated Goat Anti-Rabbit IgG antibody (green, orb868805) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Ki-67) Polyclonal Antibody, Unconjugated (orb13523) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Human stomach), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Ki-67) Polyclonal Antibody, Unconjugated (orb13523) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human ovarian serous carcinoma, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Ki-67 Polyclonal Antibody, Unconjugated (orb13523) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Tonsil, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Ki-67 Polyclonal Antibody, Unconjugated (orb13523) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.