You have no items in your shopping cart.
Ki67 Recombinant Rabbit Monoclonal Antibody
SKU: orb2563513
Featured
Description
Images & Validation
−Item 1 of 15
| Tested Applications | FC, ICC, IF, IHC-Fr, IHC-P, WB |
|---|---|
| Dilution range | IHC-P=1:200-1000, IHC-F=1:200-1000, ICC/IF=1:200-1000, IF=1:200-1000, WB=1:1000-5000, Flow-Cyt=1:100-200 |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Mouse, Rat |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Recombinant |
| Isotype | IgG |
| Clone No. | D9H1 |
| Immunogen | KLH conjugated synthetic peptide derived from human Ki67 |
| Target | MKI67 |
| Molecular Weight | 358 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Disclaimer | For research use only |
Alternative Names
−Antigen identified by monoclonal antibody Ki 67; Antigen KI67; KIA; MKI67; Proliferation related Ki 67 antigen; Antigen KI-67; KI67_HUMAN.
Similar Products
−- Item 1 of 13
Ki67 Recombinant Rabbit Monoclonal Antibody [orb1499263]
FC, ICC, IF, IHC-Fr, IHC-P
Mouse, Rat
Human, Mouse, Rat
Rabbit
Recombinant
Unconjugated
50 μl, 100 μl, 25 μl - Item 1 of 5
Rabbit anti-Ki-67 Recombinant Monoclonal Antibody [orb1519947]
FC, ICC, IF, IHC
Human
Rabbit
Recombinant
Unconjugated
10 μl - Item 1 of 5
Rabbit anti-Ki-67 Recombinant Monoclonal Antibody [orb1519949]
FC, ICC, IF, IHC
Human
Rabbit
Recombinant
Unconjugated
100 μl 
Recombinant Ki-67 (Proliferating Cell Marker) Antibody [orb751387]
IHC, WB
Human
Rabbit
Monoclonal
Unconjugated
100 μg (without BSA and Azide), 100 μg, 20 μgRecombinant Ki-67 (Proliferating Cell Marker) Antibody [orb751385]
IHC, WB
Human
Rabbit
Monoclonal
Unconjugated
100 μg (without BSA and Azide), 100 μg, 20 μg
Quality Guarantee
Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.
4% Paraformaldehyde-fixed Hela (H) cell, Triton X-100 at r.t. for 20 min, Antibody incubation with (Ki-67) monoclonal Antibody, unconjugated (orb2563513) 1:200, 90 min at 37°C, followed by conjugated Goat Anti-Rabbit IgG antibody (green, orb868805) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
(Negative control) Paraformaldehyde-fixed, paraffin embedded Mouse Herat, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Ki67 Monoclonal Antibody, Unconjugated (orb2563513) at 1:500 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
(Negative control) Paraformaldehyde-fixed, paraffin embedded Rat Herat, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Ki67 Monoclonal Antibody, Unconjugated (orb2563513) at 1:500 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Ki67Monoclonal Antibody, Unconjugated (orb2563513) at 1:500 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Ki67Monoclonal Antibody, Unconjugated (orb2563513) at 1:500 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Gastric Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Ki67Monoclonal Antibody, Unconjugated (orb2563513) at 1:500 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Ovarian Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Ki67Monoclonal Antibody, Unconjugated (orb2563513) at 1:500 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Spleen, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Ki67Monoclonal Antibody, Unconjugated (orb2563513) at 1:500 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Tonsil, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Ki67Monoclonal Antibody, Unconjugated (orb2563513) at 1:500 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Colon, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Ki67Monoclonal Antibody, Unconjugated (orb2563513) at 1:500 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Spleen, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Ki67Monoclonal Antibody, Unconjugated (orb2563513) at 1:500 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. PBS instead of the primary antibody was used as the blank control.
Paraformaldehyde-fixed, paraffin embedded Rat Colon, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Ki67Monoclonal Antibody, Unconjugated (orb2563513) at 1:500 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat Liver, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Ki67Monoclonal Antibody, Unconjugated (orb2563513) at 1:500 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat Skin, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Ki67Monoclonal Antibody, Unconjugated (orb2563513) at 1:500 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat Spleen, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Ki67Monoclonal Antibody, Unconjugated (orb2563513) at 1:500 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Quick Database Links
Gene Symbol
MKI67
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
Request a Document
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
FC
Flow Cytometry
IF
Immunofluorescence
ICC
Immunocytochemistry
Ki67 Recombinant Rabbit Monoclonal Antibody (orb2563513)
- 0.0
Based on 0 reviews
Participating in our Biorbyt product reviews program enables you to support fellow scientists by sharing your firsthand experience with our products.
Login to Submit a ReviewAvailable Sizes
Select a size below
Usually dispatched within 5-10 working days