Kras Rabbit Polyclonal Antibody

SKU: orb582746

Description

Rabbit polyclonal antibody to Kras

Research Area

Epigenetics & Chromatin, Stem Cell & Developmental Biology

Images & Validation

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Item 1 of 2

Tested Applications	WB
Reactivity	Rat
Predicted Reactivity	Bovine, Canine, Equine, Guinea pig, Human, Mouse, Rabbit, Zebrafish

Related Conjugates & Formulations

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Biotin

FITC

HRP

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Target	Kras
Molecular Weight	20kDa
Purification	Affinity Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration	0.5 mg/ml
Disclaimer	For research use only

Alternative Names

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p21, Kras2, c-Ki-ras

Quality Guarantee

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WB Suggested Anti-Kras Antibody, Titration: 1.0 ug/ml, Positive Control: Rat Lung.

Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/ml in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.

Quick Database Links

Gene Symbol

Kras

UniProt

A0JN17

RefSeq (Protein):NP_113703

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_113703

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Protocol Information

Western Blot (IB, immunoblot)

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