LEF1 Rabbit Polyclonal Antibody

SKU: orb574185

Description

Rabbit polyclonal antibody to LEF1

Research Area

Cell Biology, Epigenetics & Chromatin, Molecular Biology, Stem Cell & Developmental Biology

Images & Validation

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Item 1 of 3

Tested Applications	IHC, WB
Reactivity	Human
Predicted Reactivity	Bovine, Canine, Equine, Goat, Guinea pig, Mouse, Rabbit, Rat, Zebrafish

Related Conjugates & Formulations

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Biotin

FITC

HRP

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Immunogen	The immunogen is a synthetic peptide directed towards the C terminal region of human LEF1
Target	LEF1
Protein Sequence	Synthetic peptide located within the following region: VKPQHEQRKEQEPKRPHIKKPLNAFMLYMKEMRANVVAECTLKESAAINQ
Molecular Weight	44kDa
Purification	Protein A purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration	1.0 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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LEF-1, TCF10, TCF7L3, TCF1ALPHA

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Positive control (+): Human Ovary (OV), Negative control (-): Human liver (LI), Antibody concentration: 3 ug/ml.

Human Intestine

WB Suggested Anti-LEF1 Antibody Titration: 2.0 ug/ml, Positive Control: Jurkat cell lysate, LEF1 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells.

Quick Database Links

Gene Symbol

LEF1

UniProt

Q9UJU2

RefSeq (Protein):NP_057353

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_057353

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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