LRP1 Rabbit Polyclonal Antibody

SKU: orb6328

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Description

Images & Validation

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Item 1 of 4

Tested Applications	FC, IF, IHC-Fr, IHC-P
Dilution range	IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1μg /test
Reactivity	Human, Mouse
Predicted Reactivity	Bovine, Canine, Porcine, Rabbit, Rat, Sheep

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human LRP1 (4451-4545/4545aa)
Target	LRP1
Molecular Weight	85/515 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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A2MR; alpha 2 macroglobulin receptor; alpha 2MR; APOER; Apolipoprotein E receptor; APR; CD 91; CD91; CD91 antigen; Low density lipoprotein receptor related protein 1; Low density lipoprotein related protein 1; LRP 1; LRP 515; LRP 85; LRP; LRP ICD; LRP1 protein; LRP515; LRP1_HUMAN.

Quality Guarantee

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Blank control (blue): U937 (fixed with 2% paraformaldehyde (10 min). Primary Antibody: Rabbit Anti-LRP1 antibody (orb6328), dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), dilution: 1:200 in 1X PBS containing 0.5% BSA.

Sample: Lane 1: A549 (Human) Cell Lysate at 30 ug, Lane 2: HepG2 (Human) Cell Lysate at 30 ug, Lane 3: U2os (Human) Cell Lysate at 30 ug, Primary: Anti-LRP1 (orb6328) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 85 kD, Observed band size: 85 kD.

Tissue/Cell: Mouse brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-LRP1 Polyclonal Antibody, Unconjugated (orb6328) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/Cell: Mouse lung carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-LRP1 Polyclonal Antibody, Unconjugated (orb6328) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

LRP1

UniProt

Q07954

UniProt Details

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No UniProt data available

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Protocol Information

IHC-P

Immunohistochemistry Paraffin

View Protocol

IHC-Fr

Immunohistochemistry Frozen

Flow Cytometry

Immunofluorescence