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LwCas13a
SKU: orb1742853
Description
Images & Validation
−Item 1 of 2
Key Properties
−| Source | Leptotrichia wadei |
|---|---|
| Biological Activity | It is recommended that the researcher perform a serial dilution of Active Cas13 diluted with 1X Reaction Buffer for optimal result |
| Tag | His |
| Molecular Weight | Calculated MW: 139.8 kDa. Observed MW: ~125 kDa |
| Purity | The purity of LwCas13a, Active was determined to be >70% by densitometry |
Storage & Handling
−| Storage | Store at –70oC. To avoid repeated handling and multiple freeze/thaw cycles aliquot product into smaller quantities |
|---|---|
| Form/Appearance | Recombinant protein stored in 25 mM Tris, pH 7.5, 300 mM NaCl, 0.1 mM TCEP, 50% Glycerol. |
| Buffer/Preservatives | Recombinant protein stored in 50mM sodium phosphate, pH 8.0, 300mM NaCl, 150mM imidazole, 1mM DTT, 10% glycerol.. Recombinant protein stored in 50mM sodium phosphate, pH 8.0, 300mM NaCl, 150mM imidazole, 1mM DTT, 10% glycerol. |
| Concentration | 0.1 μg/μl |
| Dry Ice Shipping | Please note: This product requires shipment on dry ice. A dry ice surcharge will apply. |
| Disclaimer | For research use only |
Alternative Names
−LwaCas13a, LwC2c2, EndoRNase, CRISPR-associated endoribonuclease Cas13a
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The purity of LwCas13a, Active was determined to be >70% by densitometry. Calculated MW: 139.8 kDa. Observed MW: 125 kDa
LwCas13a nuclease activity assayed using a fluorogenic substrate and 5nM of enzyme as per the Cas13 Activity Assay
Quick Database Links
UniProt
UniProt Details
− No UniProt data available
Protocol Information
LwCas13a (orb1742853)
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