MBD4 Rabbit Polyclonal Antibody

SKU: orb329796

Description

Rabbit polyclonal antibody to MBD4

Research Area

Epigenetics & Chromatin, Molecular Biology, Signal Transduction, Stem Cell & Developmental Biology

Images & Validation

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Item 1 of 2

Tested Applications	ChIP, WB
Reactivity	Human
Predicted Reactivity	Guinea pig

Related Conjugates & Formulations

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Biotin

FITC

HRP

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Immunogen	The immunogen is a synthetic peptide directed towards the middle region of human MBD4
Target	MBD4
Molecular Weight	66kDa
Purification	Affinity Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration	0.5 mg/ml
Disclaimer	For research use only

Alternative Names

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anti MED1 antibody

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Quiescent human colon carcinoma HCT116 cultures were treated with 10% FBS for three time points (0, 15, 30min) or (0, 30, 60min) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site.

WB Suggested Anti-MBD4 Antibody Titration: 0.2-1 ug/mL, ELISA Titer: 1:12500, Positive Control: Hela cell lysate, MBD4 is strongly supported by BioGPS gene expression data to be expressed in Human HeLa cells.

Quick Database Links

Gene Symbol

MBD4

UniProt

O95243

RefSeq (Protein):NP_003916

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_003916

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Protocol Information

Western Blot (IB, immunoblot)

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ChIP

Chromatin Immunoprecipitation

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