MMP9 Recombinant Rabbit Monoclonal Antibody

SKU: orb704499

Featured

Description

Research Area

Cancer Research, Signal Transduction, Thrombosis, Varscular System

Images & Validation

−

Item 1 of 4

Tested Applications	IF, IHC-Fr, IHC-P, WB
Dilution Range	WB=1:2000-20000, IHC-P=1:200-1000, IHC-F=1:200-1000, IF=1:200-1000
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Mouse

Related Conjugates & Formulations

−

Key Properties

−

Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	IgG
Clone No.	7D6
Immunogen	A synthesized peptide derived from human MMP9 (100-165/707aa)
Target	Mmp9
Molecular Weight	100 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

−

Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

−

Matrix metalloproteinase-9 precursor; MMP-9; MMP9; MMP 9; 92 kDa type IV; Collagenase; 92 kDa gelatinase; Gelatinase B; GELB; MMP9_HUMAN; 82 kDa matrix metalloproteinase-9; 92 kDa type IV collagenase; CLG 4B; CLG-4B; CLG4B; Collagenase Type 4 beta; Collagenase Type-4 beta; Collagenase type IV 92 KD; Collagenase type IV 92 KD; EC 3.4.24.35; Gelatinase 92 KD; Gelatinase 92 KD; Gelatinase beta; Gelatinase-beta; GelatinaseB; GELB; Macrophage gelatinase; MANDP2; Matrix metallopeptidase 9(gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase); Matrix Metalloproteinase 9; Type V collagenase.

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

MMP9 Recombinant Rabbit Monoclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (MMP9) Monoclonal Antibody, Unconjugated (orb704499) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human tonsil), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (MMP9) Monoclonal Antibody, Unconjugated (orb704499) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Normal human U-2 OS cell lysates, Lane 2: U-2 OS treated with 200nM TPA for 48 hours, Lane 3: Normal human MDA-MB-231 cell lysates, Lane 4: MDA-MB-231 serum starved overnight, then treated with 200nM TPA for 24 hours, Primary: Anti-MMP9 (orb704499) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 100 kDa, Observed band size: 82 kDa.

Sample: Lane 1: Spleen (Rat) Lysate at 40 ug, Lane 2: Lung (Rat) Lysate at 40 ug, Primary: Anti-MMP9 (orb704499) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 92/82-85 kD, Observed band size: 90 kD.

Quick Database Links

Gene Symbol

Mmp9

UniProt

P14780

UniProt Details

−

No UniProt data available

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

Western Blot (IB, immunoblot)

View Protocol

IHC-P

Immunohistochemistry Paraffin

View Protocol

IHC-Fr

Immunohistochemistry Frozen

View Protocol

Immunofluorescence

View Protocol

Find More Protocols

Berta H Ganizada 1 2 3, Koen D Reesink 3 4, Shaiv Parikh 3 4, Pepijn J M H Saraber 2 3 4, Jack P Cleutjens 3 5, Austin Isabella 2 3, Mathilde Q E Krebbekx 1 3, Armand M Jaminon 2 3, Cecile F M Maassen 2 3, Mitch J F G Ramaekers 3 6 7, Bart Spronck 3 4, Koen van der Laan 3 4, Rory R Koenen 2 3, Roberto Lorusso 1 3, Jos G Maessen 1 3, Elham Bidar 1 3, Leon J Schurgers Loss of wall stress homoeostasis in ascending thoracic aortic aneurysm: histomorphometric insights into patient variants J Physiol, (2025)

PubMed: 40581980