Nestin Rabbit Polyclonal Antibody

SKU: orb500984

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Description

Research Area

Angiogenesis, Angiogenic Factors, Cancer Research, Cardiovascular Research, Cell Biology, Cell Markers, Cell Signaling, Intercellular Signaling, Intermediate Filaments, Neurogenesis, Neuronal restricted progenitors (NRPs), Neuroscience, Stem Cell Research

Images & Validation

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Item 1 of 2

Tested Applications	FC
Dilution Range	Flow-Cyt=1μg/Test
Reactivity	Mouse
Predicted Reactivity	Rat

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from rat Nestin (681-750/1893aa)
Target	NES
Molecular Weight	209 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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ESTM 46; FLJ 21841; FLJ21841; Intermediate filament protein; Nbla00170; Nes; NEST_RAT; Nestin.

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Cell: Neuro-2a, Concentration: 1:100, Host/Isotype: Rabbit/IgG, Flow cytometric analysis of Rabbit IgG isotype control on Neuro-2a (blue) compared with primary antibody (orb500984) (green) followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG (H+L) secondary antibody.

Tissue/Cell: U-251 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (Nestin) polyclonal Antibody, Unconjugated (orb500984) 1:100, 90 minutes at 37°C, followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Quick Database Links

Gene Symbol

NES

UniProt

P21263

UniProt Details

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No UniProt data available

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Protocol Information

Flow Cytometry

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