NRF1 Rabbit Polyclonal Antibody

SKU: orb575133

Description

Rabbit polyclonal antibody to NRF1

Images & Validation

−

Item 1 of 12

Tested Applications	IHC, WB
Reactivity	Human, Mouse
Predicted Reactivity	Bovine, Canine, Equine, Guinea pig, Rabbit, Rat, Zebrafish

Related Conjugates & Formulations

−

Biotin

FITC

HRP

Key Properties

−

Host	Rabbit
Clonality	Polyclonal
Immunogen	The immunogen is a synthetic peptide directed towards the middle region of Human NRF1
Target	NRF1
Molecular Weight	54 kDa
Purification	Affinity Purified
Conjugation	Unconjugated

Storage & Handling

−

Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration	0.5 mg/ml
Disclaimer	For research use only

Alternative Names

−

ALPHA-PAL

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

25 ug of the indicated Human whole cell or tissue extracts was loaded onto a 12% SDS-PAGE gel. 3 ug/ml of the antibody was used in this experiment.

Sample Tissue: Mouse Heart, Antibody dilution: 1 ug/ml.

Sample Tissue: Human A549 Whole Cell, Antibody dilution: 3 ug/ml.

Sample Tissue: Human U937 Whole Cell, Antibody dilution: 2 ug/ml.

Human 293T. There is BioGPS gene expression data showing that NRF1 is expressed in HEK293T.

Human Hela. There is BioGPS gene expression data showing that NRF1 is expressed in HeLa.

Human Jurkat. There is BioGPS gene expression data showing that NRF1 is expressed in Jurkat.

Human Prostate

Rabbit Anti-NRF1 Antibody, Catalog Number: orb575133, Formalin Fixed Paraffin Embedded Tissue: Human Adult heart, Observed Staining: Nuclear, Primary Antibody Concentration: 1:600, Secondary Antibody: Donkey anti-Rabbit-Cy2/3, Secondary Antibody Concentration: 1:200, Magnification: 20X, Exposure Time: 0.5- 2.0 sec.

Rabbit Anti-NRF1 Antibody, Catalog Number: orb575133, Formalin Fixed Paraffin Embedded Tissue: Human Adult liver, Observed Staining: Nuclear and some weak cytoplasmic, Primary Antibody Concentration: 1:600, Secondary Antibody: Donkey anti-Rabbit-Cy2/3, Secondary Antibody Concentration: 1:200, Magnification: 20X, Exposure Time: 0.5 -2.0 sec.

WB Suggested Anti-NRF1 Antibody Titration: 0.2-1 ug/ml, ELISA Titer: 1:62500, Positive Control: Transfected 293T.

WB Suggested Anti-NRF1 antibody Titration: 1 ug/ml, Sample Type: Human heart.

Quick Database Links

Gene Symbol

NRF1

UniProt

Q16656

RefSeq (Protein):NP_005002

UniProt Details

−

No UniProt data available

NCBI Reference Sequences

−

Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_005002

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

Western Blot (IB, immunoblot)

View Protocol

IHC

Immunohistochemistry

View Protocol

Find More Protocols