Orexin receptor 1+2 Rabbit Polyclonal Antibody

SKU: orb11198

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Description

Images & Validation

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Item 1 of 4

Tested Applications	IF, IHC-Fr, IHC-P, WB
Dilution range	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Reactivity	Mouse, Rat
Predicted Reactivity	Bovine, Canine, Equine, Gallus, Human, Porcine, Rabbit

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human Orexin receptor 1/2 (321-425/444aa)
Target	Hcrtr1
Molecular Weight	50 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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Orexin Receptor 1 and 2; Orexin receptor 1+2; Orexin Receptor 2; HCRTR 1; HCRTR1; Hypocretin receptor type 1; Orexin receptor type 1; Ox-1-R; Ox1-R; Ox1r; OX1R_HUMAN; Hcrtr2; Hypocretin(orexin) receptor 2; Hypocretin receptor 2; Hypocretin receptor type 2; Orexin receptor 2; Orexin receptor type 2; Ox-2-R; Ox2-R; Ox2r; OX2R_HUMAN.

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Orexin receptor 1+2 Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Orexin receptor 1+2) Polyclonal Antibody, Unconjugated (orb11198) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse Cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Orexin receptor 1+2) Polyclonal Antibody, Unconjugated (orb11198) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Cerebral cortex (Mouse) Lysate at 40 ug, Hippocampus (Mouse) Lysate at 40 ug, Brain (Mouse) Lysate at 40 ug, Brain (Rat) Lysate at 40 ug, Primary: Anti-Orexin receptor 1+2 (orb11198) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 50 kD, Observed band size: 50 kD.

Sample: Lane 1: Mouse Cerebrum tissue lysates, Lane 2: Mouse Cerebellum tissue lysates, Lane 3: Rat Cerebrum tissue lysates, Lane 4: Rat Cerebellum tissue lysates, Primary: Anti-Orexin receptor 1+2 (orb11198) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 50 kD, Observed band size: 50 kD.

Quick Database Links

Gene Symbol

Hcrtr1

UniProt

O43613

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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Find More Protocols

Sun, Haoyu et al. CD200R, a co-inhibitory receptor on immune cells, predicts the prognosis of human hepatocellular carcinoma Immunol Lett, 178, 105-113 (2016)

PubMed: 27562325